Entretanto a expressão dessas proteínas no câncer esporádico e familiar ainda Therefore, characterization of theįamilial breast cancer phenotype will improve the understanding of breast carcinogenesis.ĬONTEXTO E OBJETIVO: As proteínas p63, p-cad e CK 5 são expressas em células basais/mioepiteliaisĭa mama. CONCLUSION: Familial breast cancer was found to be associated with basalīiomarkers, using tissue microarray technology. While absence of coexpression (OR = 0.13) was associ-ated with the sporadic cancer Had coexpression of the basal biomarkers CK5+/ p63+, grouped two by two (OR = 34.34), RESULTS:įamilial tumors were more frequently positive for the p-cadherin (p = 0.0004), p63 Women at high risk were based on those of the Breast Cancer Linkage Consortium. Was per-formed on 168 samples from a breast cancer case series. METHODS: Immunohistochemistry using the antibodies p63, CK5 and p-cadherin,Īnd also estrogen receptor (ER) and Human Epidermal Receptor Growth Factor 2 (HER2), DESIGN AND SETTING: This was a cross-sectional study at Universidade Estadualĭe Campinas, Brazil, and the Institute of Pathology and Mo-lecular Immunology, Porto,
#P cad myoepithelial cells series#
Study the basal immunopro-file of a breast cancer case series using tissue microarray Basement membrane components can also be demonstrated throughout the collapsed alveoli.CONTEXT AND OBJECTIVE: The proteins p63, p-cadherin and CK5 are consistently expressedīy the basal and myoepithelial cells of the breast, although their expression in sporadicĪnd familial breast cancer cases has yet to be fully defined. The basement membrane is thicker and is occasionally incomplete, as also are the basket-like myoepithelial structures. During involution, the alveoli collapse, and appear to be in a state of dissolution. In the lactating gland, fibronectin appears to be located in the basement membrane region in addition to being a component of the stroma. The basement membrane in the lactating gland is still continuous around the ducts and alveoli.
![p cad myoepithelial cells p cad myoepithelial cells](https://ars.els-cdn.com/content/image/1-s2.0-S0012160616301300-gr7.jpg)
In the secretory alveoli of the lactating rat, the myoepithelial cells no longer appear to form a continuous layer beneath the epithelial cells and in many areas the epithelial cells appear to be in contact with the basement membrane. Only the basal or myoepithelial cells in the terminal end buds of neonatal animals demonstrate cytoplasmic staining for basement membrane proteins, indicating active synthesis of these proteins during this period.
![p cad myoepithelial cells p cad myoepithelial cells](https://www.mdpi.com/cancers/cancers-13-02815/article_deploy/html/images/cancers-13-02815-g001.png)
The ductal system is surrounded by connective tissue. Antiserum to fibronectin does not delineate the basement membrane in the resting gland. In the resting, virgin mammary gland, myoepithelial cells appear to form a continuous layer around the epithelial cells and are in turn surrounded by a continuous basement membrane. Antisera to myosin and prekeratin were used to localize myoepithelial cells, antisera to rat milk fat globule membrane for epithelial cells, antisera to laminin and type IV collagen to delineate the basement membrane and antisera to type I collagen and fibronectin as markers for connective tissue. resting, pregnant, lactating, and involuting glands. Using antisera to specific proteins, the localization of the rat mammary parenchymal cells (both epithelial and myoepithelial), the basement membrane, and connective tissue components has been studied during the four physiological stages of the adult rat mammary gland, viz.